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KMID : 1134820190480040447
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 4 p.447 ~ p.455
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Optimization of Deodeok (Codonopsis lanceolata) Extraction Condition Using Response Surface Methodology
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Kim Yi-Seul
Kim Seong-Ho
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Abstract
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The deodeok extraction conditions were optimized by a response surface methodology (RSM). The physicochemical properties and major components of the deodeok extracts were analyzed. A central composite design was applied to examine the effects of three independent variables: solvent to sample ratio (10∼30 mL/g; X1), extraction temperature (70∼90°C; X2), and extraction time (1∼5 hour; X3). The extraction conditions were optimized with the characteristics of the extracts as a dependent variable. As a result, the extraction yields were 35.01∼71.53% and the coefficient (R2) of the derived equation from the response surface regression for extraction yield was 0.9574. The total sugar and reducing sugar contents in all extracts were 3.19∼6.04 mg/mL and 1.58∼3.11 mg/mL, respectively, which were within the 1% significance level. The crude saponin contents in all extraction samples were 0.09∼0.17% with a R2 value of 0.9373 and were within the 1% significance level. The lobetyolin content as a major component in all extraction samples was 16.72∼32.82 μg/mL with a R2 value of 0.9791 that was within the 1% significance level. The optimal conditions based on the superimposing of the four-dimensional RSM on the crude saponin and lobetyolin contents under various conditions were established as follows: solvent to sample ratio, 12 mL/g; extraction temperature, 75°C; and extraction time, 3 hours.
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KEYWORD
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deodeok, Codonopsis lanceolata, extraction condition, response surface methodology
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